DNA2.0 Celebrates Freedom of Expression With Mammalian Expression Vectors

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pJmammalian Vectors Provide Superior Expression, Come Without IP Restrictions and are Free to Gene Synthesis Customers

Figure: GFP expressed from pCDNA3.1 and pJ603 (Neomycin, pUC high copy origin expression vector) shows equivalent expression levels in pJ603 as measured by both FACS and immunoflourescence. (Images: Dr. Aaron Straight, Stanford University)

Together with DNA2.0’s patented gene optimization technology, our pJmammalian and pJexpress vectors offer the most integrated and efficient path from virtual sequence to expressed protein.

DNA2.0, the leading gene synthesis and protein engineering company, today announced the availability of pJmammalian expression vectors, the latest offering in DNA2.0’s unique and powerful family of expression vectors. Along with pJexpress bacterial expression vectors, the pJmammalian family of vectors are free for gene synthesis customers and provide the flexibility of working with expression vectors that are not constrained by intellectual property restrictions. These bacterial and mammalian vector lines are available now from DNA2.0 with the same speed and PhD-level support that customers have come to expect from the world’s fastest provider of synthetic genes. DNA2.0 continues to develop new vector lines in a wide range of hosts.

“Regardless of the type of research you are engaged in, strong protein expression is your goal,” said Claes Gustafsson, Co-Founder and Vice President at DNA2.0. “Together with DNA2.0’s patented gene optimization technology, our pJmammalian and pJexpress vectors offer the most integrated and efficient path from virtual sequence to expressed protein.”

pJmammalian Features

pJmammalian vectors offer equal or higher levels of protein expression than a pCDNA3.1 vector when using the same insert. When receiving a free vector in conjunction with a gene synthesis order, DNA2.0 customers may:

  • Choose from Blasticidin, Hygromycin, Neomycin, Puromycin or Zeocin resistance markers
  • Achieve read through elimination using both a transcription terminator before the CMV promoter and a lac operator
  • Choose low copy (pACYC) or high copy (pUC) bacterial origin
  • Include Bovine Growth Hormone polyadenylation signal (BGH pA) to protect mRNA from hydrolytic enzymes and provide stability
  • Custom modify the leader and open reading frame
  • Enjoy the peace of mind of using vectors that are free from IP restrictions

IP Protection and Superior Customer Service

While many other gene synthesis companies offshore manufacturing to areas of the world with lax IP protection, all DNA2.0 genes are made at the company’s state-of-the-art facility in Menlo Park, thus ensuring the greatest security for patented or confidential sequences. DNA2.0 guarantees researchers high-quality synthetic genes, delivered on time, every time.

About DNA2.0

Founded in 2003, DNA2.0 is the leading synthetic genomics company. It is the fastest provider of synthetic genes, based in the US with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. DNA2.0 explores novel applications for synthetic genes and is exploiting the synergy between highly efficient gene synthesis process and new protein optimization technologies. The tools and applications brought to market by DNA2.0 are transforming biology into an engineering discipline. The company is privately held and is headquartered in Menlo Park, Calif. For more information, please visit http://www.DNA20.com.

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Claes Gustafsson
DNA2.0
650-853-8347
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