3D Biomatrix™ Perfecta3D™ Hanging Drop Plates Facilitate Spheroid Formation from Twenty Prominent Cell Lines

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3D Biomatrix, which develops and sells tools for drug discovery research and testing that mimic the human cellular environment, has demonstrated the use of its Perfecta3D(TM) Hanging Drop Plates to facilitate the formation of cell spheroids utilizing 20 cell lines.

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3D Biomatrix Perfecta3D Hanging Drop Plates

Formation of spheroids from cell lines from several mammals (human, mouse, monkey) demonstrates that the versatility of the Hanging Drop Plates will appeal to the majority of researchers that want to perform 3D cell culture.

3D Biomatrix, a leader in the development of three-dimensional (3D) cell culture products, and their collaborators have formed cellular spheroids from more than 20 cell lines utilizing Perfecta3D(TM) Hanging Drop Plates. The formation of spheroids from cell lines from several mammals (human, mouse, monkey) demonstrates that the versatility of the Hanging Drop Plates will appeal to the majority of researchers that want to perform 3D cell culture.

The focus of efforts by 3D Biomatrix and their collaborators has been on many carcinoma cell lines, including those sourced from human breast (BT-20, MCF-7, MDA-MB-231, SUM159), colorectal (Caco-2, HCT-116, HRT-18, HT-29), and prostate (DU-145) cancers. These, along with other carcinoma cell lines, demonstrate the use of the Hanging Drop Plates to model solid tumors.

As many researchers utilize murine cell lines, the company has demonstrated that spheroids have been formed using a mouse colon carcinoma cell line (CT26), a mouse osteoblastic cell line (MT3C3-E1), and a murine embryonic stem cell line (ES-D3).

“We are thrilled to see that drug research and discovery laboratories find our 3D Hanging Drop Plates not only easy to adopt, but also a more effective tool to grow and test different cellular spheroids in a true bio-mimicking environment – well before they get to the animal testing stage,” said Laura Schrader, CEO of 3D Biomatrix. “With our plates, innovative labs are making more confident, selective decisions on which compounds to test further. Having that impact is very rewarding for us as a company.”

The results of spheroid formation from a human epithelial carcinoma cell line (A431.H9), an African green monkey kidney fibroblast cell line (COS7), and murine embryonic cell line ES-D3, were published in the journal Analyst earlier this year. In this paper, the size of spheroids could be controlled by the number of cells initially seeded, and consistent and controllable spheroid cultures were demonstrated for up to 12 days. Spheroids were then used to compare the efficacy of several anti-cancer drugs and compare data to 2D cultures.

A complete list of cell lines used to form spheroids in the Perfecta3DTM Hanging Drop Plates is located at http://3dbiomatrix.com/2011/07/01/perfecta3d-hanging-drop-plates-cell-types/.

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