The importance of using cell-based assays as a complement to traditional analytical chemistry screening techniques has been highlighted in our collaborations with researchers seeking new solutions for detecting endocrine disrupting chemicals in recycled w
Menlo Park, CA (PRWEB) December 09, 2011
SwitchGear Genomics, Inc., a leading provider of products for studying regulatory elements in the human genome, has launched their LightSwitch Multiplex Assay Kits for environmental toxicology and water quality testing as announced at a recent meeting of water toxicology researchers. The new kits leverage SwitchGear’s validated human promoter-luciferase biomarker sets and a new multiplexed assay design that enables researchers to measure the activity of multiple biomarkers and controls in a single well. The novel kits are available for many environmentally relevant pathways including androgen, estrogen, glucocorticoid and thyroid receptor signaling in addition to assays for AhR/dioxin and DNA repair and genotoxicity.
“SwitchGear is well-positioned to serve the emerging market for bioassays in environmental testing,” said Shelley Force Aldred, Ph.D., President of SwitchGear Genomics. “The importance of using cell-based assays as a complement to traditional analytical chemistry screening techniques has been highlighted in our collaborations with researchers seeking new solutions for detecting endocrine disrupting chemicals in recycled water.”
Current water testing protocols rely on analytical chemistry techniques that identify known compounds of concern but cannot identify unknown toxic compounds or novel endocrine disruptors. In contrast, cell-based bioassays can identify any compound that affects a particular pathway, like estrogen signaling.
In collaboration with Shane Snyder at the University of Arizona, the SwitchGear team used the LightSwitch Multiplex Assay Kits to discover glucocorticoid-activating contaminants in a recycled water system and showed that purification protocols varied in the ability to remove the activity of those contaminants. Each kit contains the complete set of components required for environmental sample testing including cell culture components, a multiplex biomarker transfection mix, LightSwitch Assay Reagents, cell viability assay reagents, and analytical standards.