New Rapid Western Blotting Kits from AMRESCO, LLC Expand Primary Antibody Compatibility

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Save 4 hours or more of valuable research time with the innovative Rapid Western Blotting Kits, which are now available for use with goat, rat, mouse and rabbit primary antibodies.

Rapid Western blot of α-tubulin

Detection of α-tubulin in K562 lysate with Rapid Western Blotting Kit – Rat – SDT.

"[AMRESCO's Rapid Western Blotting Kits] are easy to use and have greatly reduced the time I have to spend doing Westerns..." - Dr. Patricia Dibello (Cleveland Clinic)

The streamlined simplicity of AMRESCO’s Rapid Western Blotting Kits for use with mouse and rabbit primary antibodies debuted last year to a welcome reception.

Dr. Patricia DiBello at the Cleveland Clinic said, “[AMRESCO’s Rapid Western Blottting Kits] are easy to use and have greatly reduced the time I have to spend doing Westerns…I have run Western blots for many years using semi-dry blotting followed by blocking and in some cases an overnight incubation with the primary antibody…I used Amresco’s Rapid Western Blotting Kit for rabbit antibodies and semi-dry transfer and I was able to substitute a short 1 hour incubation for the overnight incubation and the blots were beautiful….I highly recommend them.”

AMRESCO introduces two new Rapid Western Blotting Kits that are compatible with goat and rat primary antibodies. The innovative Rapid Western Blotting Kits reduce the time from transfer to protein detection to less than 90 minutes. AMRESCO’s proprietary Rapid Transfer Buffer is included in each kit, which enables protein transfer onto PVDF or nitrocellulose membranes using semi-dry or wet transfer in just 10 - 20 minutes. Transfer efficiency is equivalent to that obtained using the standard Towbin formulation. A 5-minute blocking step using AMRESCO’s popular RapidBlock™ Solution is then followed by a short incubation with combined primary antibody and Rapid Western Secondary HRP. The blot is then washed briefly with Rapid Wash Solution before detection with a chemiluminescent substrate, such as AMRESCO’s VisiGlo Plus™ Chemiluminescent Substrate Kit (available separately). Results are similar to those obtained by traditional blotting methods.

Rapid Western Blotting Kits effectively reduce time and handling steps without sacrificing results. The key to the kits’ success is the combination of proprietary secondary antibodies and specially formulated blocking and diluent reagents, which enhance antigen accessibility and minimize cross-reactivity. No special equipment is required for the protocol, enabling an easy and inexpensive transition to the Rapid Western system. With the addition of the two new products, AMRESCO’s Rapid Western Blotting Kits are now available for use with goat, rat, mouse and rabbit primary antibodies. Try a kit today to streamline Western blotting and take advantage of the time-savings that Amresco’s Rapid Western Blotting Kits offer.

For more information about Rapid Western Blotting Kits or any other AMRESCO products, visit http://www.amresco-inc.com or call 1-800-829-2805.

AMRESCO is a manufacturer and supplier of high quality biochemicals and reagents for molecular biology, life sciences, proteomics, histology and clinical areas of research. The company is ISO certified and has QSR compliant facilities to ensure customers receive the highest quality products.

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Jennifer Bayraktar

Deborah Neilly
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