Purification & Sepharose @ Scien.net
Bad Honnef, Germany (PRWEB) December 13, 2012
The aim of the Natural Sciences Bibliographic Web Portal including its two new Purification and Sepharose References sections is to provide comprehensive bibliographic information on the basic and applied life, earth and health sciences. While the publishers target to publish a total of over 21 million references until 2014, the site currently publishes about 5,000 new items every day. The basic information consists of basic bibliographic information while expanded references provide an additional summary of the publication's scientific content. Every bibliographic reference is tagged with up to twenty scientific keywords which cross-connects them beyond sections and categories.
The Chromatography Bibliography Section contains scientific information on chromatography which is the collective term for a set of laboratory procedures used in the separation of mixtures. This separation is based on differential partitioning between the mobile phase and the stationary phase. Preparative chromatography is used to separate the components of a certain mixture in order to purify it. Analytical chromatography is done with relatively smaller amounts of the material, and is used for measuring the relative proportions of analytes in a specific mixture. The latest content in this category is also available through a Chromatography Bibliography RSS feed. The section currently contains over 11,250 posts and is subdivided into the ten categories Acetonitrile, Cellulose, Florisil, HPLC, Ionization, Precolumn, Purification, Sepharose, Silicate, and Virus.
Within this section, the new category Purification provides scientific information on the process of physical separation, using chromatography. This process involves two or more substances of interest, which will then be separated from other substances that are considered to be contaminants. Purification techniques used in the study of biology and biochemistry include chromatography, which employs adsorption and desorption on a packed bed of a specific solid matter in order to purify various components of one feedstream. Copurification procedures are typically utilized to analyze the various interactions between proteins. The category contains over 790 newly published posts of which the very latest inclusions are also available through an Purification RSS feed. It features posts on the application of high-performance liquid chromatography to the purification of oligosaccharides containing neutral and acetamido sugars, studies on the separation and purification of 1-triacontanol from beeswax, and on studies on the purification of the ovarian oviposition-inducing factor in the laying hen.
The new category Sepharose contains scientific information on a cross-linked and beaded form of a polysaccharide polymer, which is extracted from seaweed. When used in research techniques such as various forms of chromatography, iodoacetyl functional groups can be added to selectively bind the cysteine side chains in the material. This method of adding iodoacetyl functional groups is often used in procedures that require the immobilization of peptides. Sepharose is produced by GE Healthcare and commonly used in chromatographic separations of a wide variety of biomolecules. This category contains over 750 newly published posts of which the very latest inclusions are also available through a Sepharose RSS feed. It features posts on radioimmunoassay of oestradiol-17 beta in cows milk after its extraction on sepharose-coupled antiserum, purification of calcium-binding proteins and peptides using phenyl-Sepharose, and on isolation of an amylase inhibitor from Setaria italica grains by affinity chromatography on blue-sepharose.
While the publishers of the web portal have a stock of 21,238,124 references of which 10,564,269 (50%) contain a summary, this information will be made successively available to the public until 2014. While the site has presently published a total of 1,314,000 posts online, the number of published bibliographic resources increases by 5,000 items every day. All items are interconnected with over 300,000 tags. The publishing status is reflected in the site's continuously updated tagline.