Unlike standard end-point cytotoxicity assays, the highly sensitive and quantitative ECIS® approach provides the entire story, not just the final chapter.
(PRWEB) February 27, 2012
Validated in vitro toxicology testing needs to be GCCP compliant. Because end point assays fail to characterize cell behavior prior to exposure they must be combined with other assays and QA/QC methods to be GCCP compliant. ECIS® assays continuously monitor cell behavior before and after exposure to compounds, thereby simultaneously providing both QA/QC and toxicology information.
Applied BioPhysics will showcase its newest advances in impedance-based toxicology measurements at the Society of Toxicology 2012Annual Meeting at the Moscone Convention Center in San Francisco, March 12-14, 2012.
At this event Applied BioPhysics, Inc. Vice President of Life Sciences, Christian W. Renken, PhD, will give the following presentation on how to use ECIS technology to implement cell based assays for toxicology screening:
ECIS: A Label Free, Non-Invasive Method of Cell Measurement
Wednesday, March 14, 2012 from 9:45 AM – 10:45 AM in Room 105
Electric Cell-substrate Impedance Sensing (ECIS®) label-free assays continuously monitor the viability of cells exposed to toxicants. ECIS® measurements allow one to follow cellular responses prior to cell death, detect effects of compounds at sub-lethal doses and monitor cell recovery after removal of toxicants.
The effects of toxic compounds upon cell migration will also be reported at the meeting using ECIS® in two different modes. The first mimics the traditional scrape wound-healing assay, but now both wounding and monitoring the healing process is accomplished electrically. In the other just released assay, cells exposed to an “electric fence” are prevented from attaching upon the ECIS® electrode, while a confluent layer forms around the electrode. When the “electric fence” is turned off, cells outside the electrode migrate in to fill the open space, and their progress is followed electrically.
Unlike standard end-point cytotoxicity assays, the highly sensitive and quantitative ECIS® approach provides the entire story, not just the final chapter.
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