Toronto, Canada (PRWEB) June 22, 2012
No single strain or vector is capable of being the best expression option for all types of biopharmaceuticals. Scientists face many expression challenges including inclusion bodies, low expression levels, and product instability. Dr. Klein will discuss how to identify the best production choice for the product of interest using Lonza’s XS tool box of experimental options, including a selection of host organisms, plasmids, promoters, selection markers, signal sequences, translational initiation regions, and stabilizing elements. Following initial strain design, samples are immediately screened using a variety of analytical techniques including SDS PAGE, Western Blot, Dot Blot, ELISA Assays, biolayer interferometry and capillary electrophoresis. Depending on the host organism, final strain selection is achieved within 6 to 8 weeks, starting from synthesized gene through to final production strain.
The 60-minute presentation will include case studies on common problems such as low productivity, product-related impurities, insolubility, plasmid and host instability.
This webinar is designed for fermentation and expression specialists with a focus in the areas of cell culture and strain development.
For more information or to register go to http://xtks.in/Plsluv
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Karen Lim (416) 977-6555 ext 227