Large Structural Genomics Core Disseminates Over 125 Atomic-Resolution Protein Structures with Microlytic Products

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Microlytic announces a major milestone in the production of protein structures using its products by two structural genomics initiatives based at Albert Einstein College of Medicine.

Microlytic is pleased to announce that more than 125 Protein Data Bank depositions have originated from the use of its crystallization products at the joint crystallization core facility of the New York Structural Genomics Research Center (NYSGRC) and the Enzyme Function Initative (EFI).

The discipline of protein crystallography centers on the generation of typically microscopic proteins in crystalline form, which are in turn analyzed under the lens of intense X-ray beams. The resulting high-resolution structure of a protein can yield a tremendous wealth of information regarding its function in both health and disease state.

"The Almo lab handles crystallization trials for the New York Structural Genomics Research Consortium (NYSGRC, part of PSI-Biology), the Enzyme Function Initiative (EFI) as well as serves as a core facility to the Einstein community and beyond,” says Rafael Toro, Manager of Crystallization Operations for the Almo Laboratory at the Albert Einstein College of Medicine. “For all of these projects we have adopted the MCSG screens as our initial screening regiment. The 384-condition screening suite covers a broad spectrum of chemical space without redundancies and has proven to be very effective in producing diffraction quality crystals, frequently without the need for further optimization. We have also found these screens have proven to be very cost effective in comparison to other commercially available screens.”

As a major contributor to the field of high throughput structural biology, the NYSGRC research effort has provided significant insight into a wide variety of important biological and biomedical problems and has contributed more than 1000 atomic resolution protein structures to the general scientific community. In its current funding phase under the Protein Structure Initiative, the NYSGRC is tackling numerous challenging and high priority targets that require enhanced experimental and computational efforts. This includes the adoption of the MCSG Crystallization Suite from Microlytic.

The MCSG Suite has been in use at the EFI since its inception in 2011. This Center aims to develop a robust strategy to integrating sequence and structural information with enzymology, genetics and metabolomics to provide functional information for enzymes without known in vivo roles. In a relatively short time, this consortium of 14 researchers from 9 academic institutions have contributed more than 80 high resolution crystallographer structures that have provided tremendous insight into the functions of both bacterial and mammalian proteins.

The MCSG Crystallization Suite was designed by researchers at the Midwest Center for Structural Genomics at Argonne National Laboratory under the oversight of Dr. Andrzej Joachimiak and is now in use at many academic and pharmaceutical research laboratories worldwide. Over 100 additional protein structures have directly resulted from use of the crystals grown using the Suite at the Argonne location, with many more contributed by other Microlytic clients.

Dr. Toro offered further praise for Microlytic’s services: “Our customer support has also been exemplary, making bulk ordering and shipping painless."

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Morten Sommer
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