(PRWEB) February 01, 2013
Validated in vitro toxicology testing needs to be good cell culture practice (GCCP) compliant. Because end point assays fail to characterize cell behavior prior to exposure they must be combined with other assays and quality assurance and quality control methods to be GCCP compliant. ECIS® assays allow the ability to continuously monitor cell behavior before and after exposure to compounds.
Electric Cell-substrate Impedance Sensing (ECIS®) label-free assays continuously monitor the viability of cells exposed to toxicants. ECIS® measurements allow one to follow cellular responses prior to cell death, detect effects of compounds at sub-lethal doses and monitor cell recovery after removal of toxicants.
Applied BioPhysics will showcase its newest advances in impedance-based toxicology measurements at the Society of Toxicology 2013 Annual Meeting San Antonio, March 10-14, 2013.
At this event Applied BioPhysics, Inc. Vice President of Life Sciences, Christian W. Renken, PhD, will give the following presentation on how to use ECIS technology to implement cell based assays for toxicology screening:
ECIS: A Label Free, Non-Invasive Method of Cell Measurement
Tuesday, March 12, 2013 from 12:15 to 1:15pm in Room 0007C
The effects of toxic compounds upon cell migration will also be reported at the meeting using ECIS® in two different modes. The first mimics the traditional scrape wound-healing assay, but now both wounding and monitoring the healing process is accomplished electrically. In the other just released assay, cells exposed to an “electric fence” are prevented from attaching upon the ECIS® electrode, while a confluent layer forms around the electrode. When the “electric fence” is turned off, cells outside the electrode migrate in to fill the open space, and their progress is followed electrically.
Unlike standard end-point cytotoxicity assays, the highly sensitive and quantitative ECIS® approach provides the entire story, not just the final chapter.
For more information contact
Christian Dehnert, VP Marketing
Applied BioPhysics, Inc.
185 Jordan Road
Troy, NY 12180