Toronto, Canada (PRWEB) July 31, 2014
Although RNA-Sequencing is a relatively new methodology, with some of the first transcriptome-wide analyses appearing around 2008, RNA-Sequencing has rapidly evolved to become a standard assay for RNA measurement. In the last several years, assays have improved to be high throughput, more specific, work with smaller input amounts, process degraded RNA, and perform well with special tissues (eg, blood samples). On the bioinformatics side, there have been improvements in references, alignment, quantitation, and variant detection methods, and the development of specialized methods, such as transcriptome assembly, fusion discovery, infectious agent detection, and metagenomic expression.
Viewers will learn about the many RNA-Sequencing related improvements, as well as some of the current limitations in inferences and precision. Some examples of improvements and new capabilities that will be discussed include:
- strand precision
- the ability to measure RNA from a small number of cells
- new protocols for degraded RNA
- the ability to sequence samples at great depths (up to 1B paired reads) extending the dynamic range to over 6 orders of magnitude
The presenter, Dr. Wendell Jones, Global Head of Genomic Bioinformatics at EA | Quintiles, will also examine some current limitations including that due to specific biases inherent to each protocol.
This webinar is part of a 5-part series on Genomics Know-How®. To register for this session or learn more about the series visit: Genomic-Know-How.
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