DNA Genotek’s Oragene(R)•RNA Kit Selected by VITO to Measure Variability in Gene Expression Before and After Stressful Events

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Saliva-Based RNA Collection Product Facilitates Investigation into Human Stress Experienced in Ground Based Facilities Mimicking Spaceflight Conditions

saliva RNA, oragene

Stefan Schneider & Vera Brümmer - Institute of Neurosciences, German Sport University Cologne provide a saliva sample with Oragene•RNA prior to parabilic flight.

These initial results convince us to intensify our work using saliva and Oragene•RNA and to explore its value in other research projects.

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DNA Genotek, a leading provider of products for biological sample collection, stabilization and preparation, today announced that VITO (Flemish Institute for Technological Research), has selected Oragene•RNA for a series of biomonitoring research projects. Human biomonitoring campaigns study the impact of environmental stressors (pollutants, irradiation, psychological stress, etc.) on health and well-being by measuring a suite of biomarkers. Gene expression has proven to be useful to identify molecular changes in blood that relate to environmental stress. VITO will explore the value of saliva gene expression in biomonitoring and investigate the potential to develop new saliva biomarkers.

The Oragene•RNA Kit is the only all-in-one system for the collection, stabilization and transportation of high quality mRNA from saliva. It is designed for human mRNA expression profiling. VITO has chosen Oragene•RNA for the following proof-of principle projects:

1. Study of the normal variability in gene expression in a healthy population and comparison with blood gene expression;
2. Effect of confinement on the neuro-immune system during an extended stay at the Concordia Research Station on the Antarctic Plateau. The project is supported by the Belgian government and is in conjunction with the Institute of Neurosciences at the German Sport University Cologne and the European Space Agency (ESA);
3. Effect of stress on human volunteers participating in parabolic flight [in conjunction with the German Sport University Cologne and ESA]. Saliva samples will be taken before and after the experiment.

Patrick De Boever, Ph.D., will lead the investigation at VITO and will advance the understanding on how stressful events might alter gene expression patterns. “We have already collected and extracted mRNA from saliva samples using Oragene•RNA to estimate time-dependent variation and intra-individual variation. We successfully processed samples taken during parabolic flights. All the samples provided high amounts of RNA and strong BioAnalyzer results and were compatible with our real-time PCR and microarray platform. These initial results convince us to intensify our work using saliva and Oragene•RNA and to explore its value in other research projects."

“The decision by VITO to use Oragene•RNA is a strong validation that our saliva-based RNA collection kit delivers leading performance,” said Ian Curry, president and CEO, DNA Genotek Inc. “Oragene•RNA’s unique ability to stabilize an RNA sample for months at ambient temperatures makes it ideal for the challenging environments in these studies.”

The studies involved in this project are the first in a range of initiatives by VITO to investigate the value of saliva for monitoring health issues during spaceflight conditions. Saliva research is now also utilized in VITO’s human biomonitoring program. More initiatives are underway to introduce the concepts in the medical and health care sector.

About DNA Genotek:
DNA Genotek focuses on providing high-quality biological sample collection products. The company’s Oragene family of products provide the best way to collect and preserve large amounts of DNA or RNA from saliva. The company also offers a family of products for collecting DNA from animals and livestock. The reliability and ease-of-use of the company’s products have resulted in rapid adoption at top-tier health research institutions globally, including Harvard, Stanford, Cambridge, and Johns Hopkins. DNA Genotek markets its products worldwide and has established a global customer base in over 100 countries. For more information about DNA Genotek, visit http://www.dnagenotek.com.

About VITO, vision on technology
VITO (Flemish institute for technological research) is a leading independent European research and consulting centre developing sustainable technologies in the area of energy, environment, materials and remote sensing. VITO provides intelligent and high quality solutions, offering a competitive advantage to large and small companies. It also provides objective research, studies and advice enabling industry and government to establish future policies. VITO’s research finds its application in sustainable process and production technologies or cleantech, environment, energy efficiency, renewable energy, smart grids, advanced material and analysis techniques, secondary materials, and healthcare. VITO counts approximately 600 highly qualified employees from diverse specialisations and collaborates with sector federations and their research centres, universities, colleges and European research institutes. For more information about VITO, visit http://www.vito.be/english.

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Shauna White