Protein Variant Library Expertise From DNA2.0

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Maximizing Screening Efficiency Through Good Library Design.

While DNA2.0‘s ProteinGPS™ system for rational protein design is the preferred approach to bioengineering when screening capacity is limiting, we are pleased to offer the flexibility and affordability of our libraries...—Claes Gustafsson, CCO

The emerging field of protein engineering offers an extraordinary opportunity to design novel proteins that can be utilized for a wide range of purposes such as creating antibody-based therapeutics or specialized enzymes for energy production or to remove toxins from the environment. By utilizing libraries to screen for desired activity through directed evolution, researchers can more quickly and efficiently engineer useful proteins. DNA2.0’s sequence libraries are powerful tools for structure-function relationship analysis or for modifying the activities of proteins or DNA regulatory regions.

DNA2.0 has developed a useful guide for maximizing screening efficiency through good library design, available in the current issue of Genetic Engineering and Biotechnology News (GEN). The tutorial guides researchers on the choice of library format based on the target application and screening capabilities available for a particular project. Properties as diverse as thermal stability, protein-ligand affinity, kinetics, substrate specificity and even catalytic mechanism can all be engineered using libraries and functional screening. Libraries may be as small as a handful of variants or as large as 10^12 variants or more.

“DNA2.0’s Protein Variant Libraries are tailored to your particular research question, whether you want to explore large, complex sequence spaces with a Combinatorial Site Library or utilize Modular libraries to create new multi-domain proteins, biochemical pathways, genetic circuits and organisms,” said Claes Gustafsson, CCO and cofounder of DNA2.0. “While DNA2.0‘s ProteinGPS™ system for rational protein design is the preferred approach to bioengineering when screening capacity is limiting, we are pleased to offer the flexibility and affordability of our libraries for our customers who are best served by high-throughput screening approaches.”

In order to make the process of designing and ordering libraries easy and seamless, DNA2.0 created Library Designer, an online tool that allows researchers the complete freedom to design libraries to their exact specification while automatically checking for sequence accuracy, diversity and features such as restriction sites. DNA2.0’s variant libraries are delivered ready for screening to identify variants with improved or altered function, which is key to engineering novel proteins or pathways. Library Designer helps to ensure that researchers get the right library for their research needs.

DNA2.0’s Library Design Specialists can help determine the best library or series of libraries to suit the needs of a particular project or research goal. DNA2.0 libraries are customized for individual research needs and are delivered with the same industry-leading speed and customer service that DNA2.0 guarantees for its complete line of bioengineering solutions.

About DNA2.0
DNA2.0 is the leading bioengineering solutions provider. Founded in 2003, DNA2.0 offers an integrated pipeline of solutions for the research community, including gene design, optimization, synthesis and cloning, as well as platforms for protein and strain engineering. It is the fastest provider of synthetic genes—based in the US with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. DNA2.0 is by far the most published synthetic gene vendor, providing expert support to and collaboration with scientists. DNA2.0 explores novel applications for synthetic genes and is exploiting the synergy between highly efficient gene design and synthesis processes and new protein optimization technologies. DNA2.0’s tools and solutions are fueling the transformation of biology from a discovery science to an engineering discipline. The company is privately held and is headquartered in Menlo Park, Calif. For more information, please visit

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