Epigentek to Offer Higher Sensitivity Next Generation Sequencing Research Products

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The advent of next-generation sequencing in recent years has lead to a rapid, increased production of genetic and epigenetic data that can be used towards development of new diagnostics, treatments, and drugs. Epigentek, a leading company specializing in epigenetics, has developed a series of products specifically designed for optimal gathering of data in next-gen sequencing applications in a more convenient manner than currently used methods.

DNA methylation

An example of an Epigentek product for DNA methylation studies.

Using next-gen sequencing techniques [...] could help researchers understand how "glitches" in the epigenome can lead to diseases such as cancer and Alzheimer’s, thereby establishing a better foundation to build treatments and diagnostics upon.

Epigentek, a Farmingdale-based company specializing in epigenetic research products, launches a series of new products for DNA library preparation used in next generation sequencing analysis of epigenetic mechanisms including genome-wide DNA methylation and DNA-protein interaction (http://www.epigentek.com/catalog/next-generation-sequencing-c-75_113.html ). The products are currently designed to be highly compatible with widely used Illumina sequencer platforms. Versions for Life Technologies’ Ion Torrent-based systems will also be available in the near future.

Next-generation sequencing is based on traditional sequencing of the precise order of nucleotides within a biological sample’s DNA, but in a lower cost, high-volume manner. Using next-gen sequencing techniques such as bisulfite-seq and ChIP-seq could help researchers understand how "glitches" in the epigenome can lead to diseases such as cancer and Alzheimer’s, thereby establishing a better foundation to build treatments and diagnostics upon.

Next-gen sequencing requires an initial preparation of a DNA library, but often necessitates a high amount of starting materials to achieve. This makes tumor biopsy and embryonic tissue samples especially challenging to sequence for epigenetic applications. Current commercial offerings from other companies are also rather expensive as well as laborious. These limitations have been addressed by Epigentek’s scientists with the development of the EpiNext(TM) series of research kits that allows for next-generation sequencing library preparation and analysis of DNA methylation and DNA-protein interaction with tiny amounts of DNA.

Specifically, Epigentek’s new kits include the EpiNext(TM) Bisulfite-Seq High Sensitivity Kit, which uses a post-bisulfite conversion DNA ligation method for highly sensitive bisulfite sequencing library construction; and the EpiNext(TM) High Sensitivity DNA Library Preparation kit, which can construct libraries from a starting DNA input of as low as 0.2 ng. Both kits contain all the necessary buffers and reagents to cover each step of DNA library preparation with the entire procedure taking less than eight hours, making them the most convenient and cost-effective methods for construction of DNA libraries used in bisulfite-seq, ChIP-seq, and MeDIP-seq.

By adding preparation tools for next-generation sequencing to its catalog, Epigentek furthers its mission to offer the most comprehensive selection of tools for researchers to study epigenetics. The EpiNext(TM) series goes hand in hand with its existing line of bisulfite conversion, chromatin immunoprecipitation, and methylated DNA immunoprecipitation products.

About Epigentek Group Inc.

Epigentek is a leading biotechnology company that focuses on developing and providing innovative technologies and products as a complete and systematic solution for epigenetic related research and drug discovery. Visit the informative website at http://www.epigentek.com.

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William Lee
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