Boston, MA (PRWEB) October 17, 2013
Industry leaders from around the world will convene in Boston, MA for Sample Prep East conference which will take place at the Hyatt Regency Cambridge December 9-10 2013. One of the many anticipated presentations will include Shashi K. Sharma, a Research Microbiologist at the U.S. Food and Drug Administration, he presents “Development of a Cell Based Functional Assay for the Detection of Botulinum Neurotoxin Type A & E.”
Shashi K. Sharma, Research Microbiologist, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration
“Development of a Cell Based Functional Assay for the Detection of Botulinum Neurotoxin Type A & E”
The standard procedure for definitive detection of BoNT-producing Clostridia is a culture method combined with neurotoxin detection using a standard mouse bioassay. The mouse bioassay is highly sensitive and specific, but it is expensive and time-consuming and there are ethical concerns due to use of laboratory animals. Cell based assays provide an alternative for mouse bioassay in screening for BoNT-producing Clostridia. Here, we describe a cell-based assay utilizing a fluorescence reporter construct with full length SNAP-25 as the linker, expressed in a neuronal cell model to study toxin activity in situ. Our data indicates that PC-12 cells have significant sensitivity to BoNT/A and E action and the assay can detect as little as 100 pM BoNT/A activity within living cells. Of the in vitro approaches published in the literature for BoNT detection, we believed that cell-based methods provide a model that more closely approximates the in vivo model and have a potential to at least reduce and refine animal assays if not replace it.
Sample Prep Conference Overview:
SAMPLE PREP EAST Conference is the 8th meeting in the Knowledge Foundation’s Sample Prep Conference Series - an internationally recognized meeting for experts in sample preparation technologies for detection, identification, diagnostics and analysis of biomedical, biological and chemical agents, substances and threats in point-of-care, laboratory and field settings will explore the latest R&D developments, ready-to-market technologies, and their applications by exploring the following topical areas:
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