Ann Arbor, MI (PRWEB) July 24, 2013
3D Biomatrix, a leader in three-dimensional (3D) cell culture products, is pleased to announce that it has filed a patent for its technology for transferring spheroids from the company’s 96-well or 384-well Perfecta3D Hanging Drop Plates to a receiving plate for analysis of the spheroids. The transfer plate designed for the 384-well Hanging Drop Plates is expected to be commercially available in 4-6 weeks.
Perfecta3D Hanging Drop Plates facilitate the formation, culture, and testing of 3D spheroids in a well plate format without the aid of coatings or matrices, and without cells coming into contact with plastic. Spheroid cultures grown in Perfecta3D Hanging Drop Plates allow preclinical researchers to easily mimic tissue metabolic and proliferative gradients, capture complex cell-matrix and cell-cell interactions, conduct co-cultures, and monitor cell growth easily and regularly.
Once spheroids form in the Hanging Drop Plates, researchers often want to transfer the 3D micro-tissues to a two-dimensional (2D) receiving plate to image and / or conduct assays. The new transfer plate acts as a funnel to transfer spheroids simultaneously from all wells of the Hanging Drop Plates to the desired receiving plate with a simple step. The receiving plate can be any 2D well plate of the researcher’s choice.
“Our goal is to help researchers save time and money by collecting more biologically relevant data,” said Laura Schrader, CEO of 3D Biomatrix. “Combining our Hanging Drop Plates with the new transfer plates make the entire process, from forming spheroids to their analysis, as easy as possible. Since our 3D cell culture products work with the equipment researchers already have in the lab, researchers no longer feel trapped in buying an entire system to achieve 3D cultures,” she said.
Spheroid formation is controllable and easy with Perfecta3D Hanging Drop Plates. Users simply pipet a cell suspension into each channel; the channel shape causes the drop to securely hang from the bottom of the well. As cells do not contact any surfaces, they aggregate into a spheroid. One spheroid forms per well, and the spheroid diameter is controlled by the cell type and number of cells added to each well. Media and compounds can be added or removed from the top of the plate without requiring new equipment, and cells can be added for co-cultures.