DNA2.0 Introduces CRISPR Tools for Genome Engineering

The revolutionary NickaseNinja™ combines a tandem pair of gRNAs in a single vector.

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Cas9 Nickase Indel Frequency

Comparison of the DNA2.0 All-in-One NickaseNinja™ vector (using tandem gRNAs and dual U6 promoters) with the conventional 2-Vector system from DNA2.0 and published data.

We strive to deliver the very best bioengineering solutions to our research customers. Our CRISPR tool set demonstrates our commitment to innovate and create more powerful tools so that our customers may more easily break ground with their discoveries.

Menlo Park, CA (PRWEB) March 04, 2014

DNA2.0 today announced the release of a set of CRISPR/Cas9 tools for genome editing and engineering. At the heart of DNA2.0’s CRISPR toolset is the revolutionary NickaseNinja system, which offers the high fidelity editing of Cas9 nickase plus guide RNA (gRNA) pairs, in a convenient single-vector format. By co-expressing two guide RNAs using dual RNA polymerase promoters, the patent-pending NickaseNinja removes the need for co-transfection of multiple vectors, improving genome editing efficiency and consistency compared with the conventional two vector systems currently available. The NickaseNinja system enables targeted mutagenesis at efficiencies over 50 percent, as compared to efficiencies around 35 percent for conventional two vector systems.

Clustered Regularly Interspaced Short Palindromic Repeats, or CRISPRs, have emerged as a next-generation genome-engineering tool because of their ability to cut genomic DNA at precise locations. This greatly simplifies the process of gene editing by allowing for rapid, efficient and precise engineering, even with complex genomes.

DNA2.0 has also created an easy-to-use gRNA Design Tool to enable optimal engineering for any target—while minimizing off-target effects—by utilizing DNA2.0’s proprietary optimization algorithms. The online, easy-to-use graphic interface enables researchers the ability to design gRNA within seconds, using gene names, loci or specific target sequences. Customers can then order the designed gRNAs as transfection ready CRISPR constructs directly from DNA2.0. DNA2.0 will clone the designed gRNAs into CRISPR vectors or customers can do the cloning work themselves by utilizing DNA2.0’s IP-Free© scarless cloning system, Electra. All of DNA2.0's CRISPR/Cas9 vectors are also available with fluorescent reporters from the IP-Free© ProteinPaintbox™ for easy visualization.

“CRISPR/Cas9 technology has simplified genome editing and enabled many exciting new approaches in a very short time,” said Jeremy Minshull, CEO and cofounder of DNA2.0. “We strive to deliver the very best bioengineering solutions to our research customers. Our CRISPR tool set, from the design tool to the NickaseNinja system, demonstrates our commitment to innovate and create more powerful tools so that our customers may more easily break new ground with their discoveries.”

About DNA2.0
DNA2.0 is the leading bioengineering solutions provider. Founded in 2003, DNA2.0 offers an integrated pipeline of solutions for the research community, including gene design, optimization, synthesis and cloning, as well as platforms for protein and strain engineering. It is the fastest provider of synthetic genes—based in the US with a global customer base encompassing academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. DNA2.0 is by far the most published synthetic gene vendor, providing expert support to and collaboration with scientists. DNA2.0 explores novel applications for synthetic genes and is exploiting the synergy between highly efficient gene design and synthesis processes and new protein optimization technologies. DNA2.0’s tools and solutions are fueling the transformation of biology from a discovery science to an engineering discipline. The company is privately held and is headquartered in Menlo Park, Calif. For more information, please visit http://www.DNA20.com.


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    DNA2.0
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