New York, NY (PRWEB) May 27, 2014
The May 2014 issue of BioTechniques (http://www.biotechniques.com) is now available as an open access journal featuring the following articles:
- Misfolded protein? We have a chemical for that
- Simultaneous isolation of mRNA and native protein from minute samples of cells
- Isolation and characterization of mammalian cells expressing the Arf promoter during eye development
- Lessons learned from vivo-morpholinos: How to avoid vivo-morpholino toxicity
- A simple filtration technique for obtaining purified human chromosomes in suspension
- Efficient construction of rAAV-based gene targeting vectors by Golden Gate cloning
- Comparative next-generation sequencing of adeno-associated virus inverted terminal repeats
- Concordance of Affymetrix GeneChip® Human Transcriptome Array 2.0 and real-time PCR results using USB® VeriQuest® qPCR master mixes
- Purification of High-quality DNA from Saliva Samples with DANAGENE Saliva System applied to TargetSeq-NGS protocols
- Sensitive ligand-based protein quantification using immuno-PCR: A critical review of single-probe and proximity ligation assays
Keywords: p19Arf; primary vitreous; perivascular cells; primary cell isolation; arf, eye development; hyaloid regression; RNA isolation and purification, enzyme assay, oligo (dT), ovary, antibodies; immunoquantitative PCR, proximity ligation assay, immuno-PCR, multiplex protein analysis, aptamers
Also available is the April 2014 issue of BioTechniques featuring the following articles:
- The twisted path to pluripotency
- Coating extracellular matrix proteins on a (3-aminopropyl)triethoxysilane-treated glass substrate for improved cell culture
- GENESUS: A two-step sequence design program for DNA nanostructure self-assembly
- Lyophilized Escherichia coli-based cell-free systems for robust, high-density, long-term storage
- Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR
- Inactivation of an integrated antibiotic resistance gene in mammalian cells to re-enable antibiotic selection
- Profile of Xiaoliang Sunney Xie - Mallinckrodt Professor of Chemistry and Chemical Biology, Harvard University
Keywords: CRISP/Cas, selectable markers, digital PCR, genomic DNA, copy number analysis, recombinate proteins, cell-free protein synthesis, proteomics, Western blots, DNA nanostructures, computational biology, DNA self-assembly, cell culture, extracellular matrix, glass slide coatings, stem cell biology, stem cell analysis, molecular biology
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BioTechniques, the international journal of life science methods, provides open access to first-quality, peer-reviewed papers on laboratory techniques and protocols. Now in its 56th volume, BioTechniques has over 80,000 print subscribers worldwide. The journal augments its peer-reviewed content with feature articles and topic-specific supplements. Visit http://www.biotechniques.com for more details or to subscribe.