Yorba Linda, CA (PRWEB) June 04, 2015
The emerging field of RNA nanotechnology has led to the utilization of ultracentrifugation in the field of bionanotechnology as sensing platforms, diverse nanoparticle construction, in vivo computing, and even in vivo drug delivery. Purification of large quantities of supramolecular RNA complexes is of paramount importance due to the large quantities of RNA needed and the purity requirements for in vitro and in vivo assays. Purification is generally carried out by liquid chromatography (HPLC), polyacrylamide gel electrophoresis (PAGE), or agarose gel electrophoresis (AGE); however, difficulties exist with each technique.
Beckman Coulter is sponsoring a new educational webinar, “Large-scale purification of RNA and RNA-based Nanoparticles by Preparative Ultracentrifugation,” which will describe an efficient method for the large-scale purification of RNA prepared by in vitro transcription using T7 RNA polymerase by cesium chloride (CsCl) equilibrium density gradient ultracentrifugation and the large-scale purification of RNA nanoparticles by sucrose gradient rate-zonal ultracentrifugation or cushioned sucrose gradient rate-zonal ultracentrifugation. The isolation technique has broad-reaching applicability as production of large quantities of RNA are critical to functional assays involving RNA splicing, RNA secondary structure, anti-sense RNA, and RNA:protein interaction studies.
Daniel Jasinski is a Graduate Research Assistant in the laboratory of Dr. Peixuan Guo, a world-renowned investigator in the area of bionanotechnology. The Guo lab has pioneered the emerging field of RNA nanotechnology, even hosting a new Gordon Research Conference in 2015 dedicated to advancing this fast growing field. Daniel’s research interests focus on the construction and manipulation of nanoparticles, composed mainly of RNA, publishing in several high-impact journals (ACS Nano, March 2014; Nucleic Acids Research, May 2014; ACS Nano, June 2014; Methods in Molecular Biology, March 2015). The clinical translation of RNA nanotechnology is hindered by the amounts of purified material needed for treatment. Confronted with this problem, Daniel recently spent time developing approaches for the large-scale production and purification of RNA nanoparticles, especially ultracentrifugation, which acted to retain the integrity and function of the nanostructures (Methods in Molecular Biology; May 2015).
The complementary webinar, hosted by LabRoots, will be presented on June 25, 2015, at 8:00am PT, 11:00am ET, 3:00pm GMT.
For full details and free registration, click here.
About Beckman Coulter:
Beckman Coulter Life Sciences’ mission is to improve the health of people around the world. A global leader in research automation, flow cytometry, centrifugation, cellular analysis, genomics products, particle characterization and capillary electrophoresis, they provide sophisticated tools for basic biological research, including: causes of disease; potential new drug development and advancing clinical research. Beckman Coulter Life Sciences delivers the highest quality solutions and customer support — a commitment to excellence that’s evident in everything they do.
LabRoots is the leading scientific social networking website and producer of online educational events and webinars, and is a powerful advocate in amplifying global networks and communities, and contributing to the advancement of science through content sharing capabilities and encouraging group interactions.
Founded in 2008, LabRoots emphasizes digital innovation in scientific collaboration and learning. LabRoots has become a primary source for current scientific news, webinars, virtual conferences and more. Join for free and become part of the largest scientific learning community in the world.