Innovative Application for Tissue Culture Grade Water Soothes Summer Dry Eye Says Fresh Water Advocate Sharon Kleyne

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Sharon Kleyne Hour Power of Water radio host announces first consumer product containing tissue culture grade laboratory water. Kleyne will discuss tissue culture grade water and summer dry eye on her Sharon Kleyne Hour™ Power of Water® radio broadcast of July 27, 2015.

“Tissue culture grades water” has been around for decades and is well known to every laboratory researcher involved in growing live tissue cell cultures. Radio host and Bio-Logic Aqua® Research founder Sharon Kleyne recently announced the first ever use of tissue culture grade water as a mass-market product ingredient. The product is Nature’s Tears® EyeMist®, a personal hand-held humidifying device for summer dry eye from Bio-Logic Aqua® Research. Tissue culture grade water is the product’s sole ingredient.

Kleyne will discuss tissue culture grade water and summer dry eye on her Sharon Kleyne Hour™ Power of Water® radio broadcast of July 27, 2015. For the live show or a podcast, go to

The syndicated Sharon Kleyne Hour™ Power of Water® radio show, hosted by Kleyne, is heard weekly on VoiceAmerica and Apple iTunes. The education oriented show is sponsored by Bio-Logic Aqua® Research, founded by Kleyne and specializing in fresh water, the atmosphere, accelerated moisture evaporation and dehydration. Nature’s Tears® EyeMist® for dry eyes is the Research Center’s signature product for dry eye.

Summer dry eye symptoms, according to Kleyne, tend to occur when temperatures are hot, humidity is low and air pollution is high. The combination greatly increases the rate at which water evaporates from the delicate tear film covering the surface of the eye, which should be 99 percent water. The one-percent that is not water - salt, beneficial proteins, antibodies and lipids (oils) - does not evaporate and becomes overly concentrated when the water evaporates.

Kleyne’s solution for humidifying ocular surfaces affected by summer dry eye was to apply a pure all-water mist to the air in front of the eyes, allowing the eyes to naturally absorb as much airborne moisture as they need – an amount usually measured in nanoliters. In seeking an ideal water to convert to a mist to benefit dry eyes, Kleyne zeroed in on “tissue culture grade water,” also called “cell culture grade water.”

The specialized water, Kleyne explains, is normally used to reconstitute powdered cell growth medium for laboratory cell cultures. For optimal cell growth, the water must have several specifically defined qualities. Above all, the waters’ attributes must not vary or it could skew the results.    

According to Julia Oxford, PhD, a molecular biologist at Boise State University, nearly 100 percent of tissue culture grade water used in laboratory research is artificially manufactured. To remove impurities, regular tap water is micro-filtered, undergoes reverse osmosis and deionization and is sterilized

Tissue-culture grade water is high grade water with no impurities or contaminants, explains Oxford. The pH of tissue-culture grade water should be slightly acidic, between 5.7 and 7.0. Inorganic minerals, essential for cell growth, must be present in minute quantities. Organic matter is absent from tissue-culture grade water. Microorganisms such as bacteria, viruses and algae are absent, as are organic breakdown products.

When Oxford tested the Trade Secret water from Bio-Logic Aqua® Research’s exclusive water source, she found that it met or exceed all criteria for tissue culture grade water. Furthermore, the analysis was more consistent than manufactured water. To Oxford’s knowledge, no all-natural water had ever before been found that meets these standards.

“I knew our Trade Secret water was special,” says Kleyne, “but I never suspected it was that special. When you think about it, though, water that is ideal for growing live human tissue cells in the laboratory should also be ideal for maintaining healthy eyes and skin.”

Dry eye symptoms include general eye discomfort, itching and burning eyes, blurred vision, watery eyes, headaches, fatigue and stress. For more information about Nature’s Tears® EyeMist®, go to

©2015 Bio-Logic Aqua® Research. All rights reserved.

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Mikaylah Roggasch
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