Worcester, Mass (PRWEB) January 13, 2016
Until the creation of the KBioBox Off-Target Analysis (OTA) tool, no prediction tool has yet proved to be consistently accurate.
The CRISPR/Cas9 system has revolutionized gene editing in biologic systems and promises to bring amazing breakthroughs and advances in basic science, biotechnology and biomedical research. However, off-target interactions are a serious drawback to using the technology and pose considerable threat to the advancement of many applications.
To determine off-target interactions, Tsai et al. recently introduced the GUIDE-seq method, which is now the fields gold standard. Multiple computational groups have since built off-target prediction tools based on GUIDE-seq parameters. However, until the creation of the KBioBox off-target analysis (OTA) tool, no prediction tool has yet proven to be consistently accurate.
KBioBox's OTA tool predicted 96.6% of the off-targets reported by the GUIDE-seq method. By comparison, MIT's Crispr design tool (http://crispr.mit.edu) and the E-CRISP tool (http://www.e-crisp.org/E-CRISP/) both found less than 50% of total off-targets reported by GUIDE-seq.
Time Efficient Analysis.
The KBioBox OTA tool provides accuracy and short run times. KBioBox's OTA runs analysis in under two minutes. Using the KbioBox Bio-Design tool in conjunction with the KbioBox OTA tool returns the "top ten" CRISPR designs, each with a complete genome off-target analysis in under ten minutes
****See the document attached for full comparison graphical data****
KBioBox is a revolutionary, bio-computational company focused on empowering next generation genetic research. Using a unique computational system driven by powerful proprietary algorithms KBioBox’s services provide rapid genome analysis illuminating the intricacies of genetic structure. By providing our clients with next level information quickly and accurately KBioBox strives to provide exceptionally valuable support to genetic researchers (Corporate and Institutional) and accelerate the time required to reach specific goals.
The most powerful nuclease Off Target Analysis available, with the ability to handle any number of mismatches. Originally designed and built especially for CRISPRs, the technology has been extended to TALENS and proprietary gene editing chemistries, and can be customized for any gene editing technique.
Quickly identify ideal target sites for gene editing, scanning entire genomes instead of small localized regions. Analysis includes verification from KBioBox's Off Target Analysis.
Quickly identify all species present in a sample, down to the sub-species. This tool can also be used to report species sharing homologous regions.
Securely store your reference sequences on our system for easy integration with our tools.
Disclaimer. The data provided represents the test results obtained under trial conditions for comparative analysis. Results may vary under different conditions.