KbioBox Launches Advanced gRNA BioDesign Tool For Optimum Gene Editing Design.

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Today's launch of KbioBox's state of the art gRNA BioDesign tool for optimal Gene Editing highlights the value of the company's proprietary algorythms in providing key data empowering next generation genetic research.

Today's launch of KbioBox's state of the art gRNA BioDesign tool for optimal Gene Editing highlights the value of the company's proprietary algorythms in providing key data empowering next generation genetic research.

Introduction.

The CRISPR/Cas9 system has revolutionized gene editing in biologic systems and promises to bring hitherto unimaginable breakthroughs and advances in basic science, biotechnology, and biomedical research. However, off-target interactions are a serious drawback to using the technology and pose considerable threat to the advancement of many applications.

In an effort to mitigate these issues, KBioBox has developed a new gRNA BioDesign tool that generates optimal gene edit designs to target specific genes, while minimizing off-target interactions. The tool designs for standard gene editing chemistries such as CRISPR/Cas9 and TALEN, and extended to design custom and proprietary enzymes.

To use the KbioBox tool, simply select a reference sequence and a coding region. The tool then rapidly generates several optimal design possibilities (e.g. several 23 base pair gRNA’s for a Cas9 application), and displays the results in an intuitive visualization containing exon/annotation information. Advanced options allow the user to restrict the decision to discrete sections of a coding region (e.g. within the first three exons). Each generated BioDesign is accompanied by a full genome off-target analysis.

*KbioBox system highlights.

Fast turnaround – Our Biodesign tool quickly identifies the top ten designs, and generates a detailed off-target analysis report for each design

Customizable – Any reference sequence or chemistry can be quickly added to the system (e.g. TALEN, and extended to design custom and proprietary enzymes). Additional design requirements are easily incorporated into the process.

Integratable – Job submission form and reports are easily embedded into exising websites or pipelines.

Extensible – Customize reports and forms with additional information pertinent to your project, to help simplify your decisions process.

**See the attached white paper for user interface details.

About KBioBox

KBioBox is a revolutionary, bio-computational company focused on empowering next generation genetic research. Using a unique computational system driven by powerful proprietary algorithms KBioBox’s services provide rapid genome analysis illuminating the intricacies of genetic structure. By providing our clients with next level information quickly and accurately KBioBox strives to provide exceptionally valuable support to genetic researchers (Corporate and Institutional) and accelerate the time required to reach specific goals.

KbioBox Services.

Off-Target Analysis.
The most powerful nuclease Off Target Analysis available, with the ability to handle any number of mismatches. Originally designed and built especially for CRISPRs, the technology has been extended to TALENS and proprietary gene editing chemistries, and can be customized for any gene editing technique.

BioDesign.
Quickly identify ideal target sites for gene editing, scanning entire genomes instead of small localized regions. Analysis includes verification from KBioBox's Off Target Analysis.

Species Identification.
Quickly identify all species present in a sample, down to the sub-species. This tool can also be used to report species sharing homologous regions.

Sequence Storage.
Securely store your reference sequences on our system for easy integration with our tools

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Tony Acuifalo

Kryngle Daly
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