“When a Western blot experiment does not produce the expected results, troubleshooting the cause of the problem can be rather challenging...the TruStrip WBV product can provide all the answers and perform accurate quantification" added Dr. Ali.
San Antonio, Texas (PRWEB) October 11, 2016
Alpha Diagnostic International, Inc. (ADI) is excited to announce the introduction of TruStrip® Western Blot Validation & Quantification (WBV) system. The TruStrip WBV system is a smart, visual, reliable, and cost-effective technology for performing on-the-spot validation of Western blot process steps and reagents, and enabling accurate quantification of proteins of interest. Individual test strips of multiple zones containing specific reagents applied as stripes provide a simple, visual result when used along with any Western blot membrane during the processing steps. For further details on TruStrip WBV technology and ordering details, please visit http://www.trustrips.com.
Western blot remains as one of the most commonly used analytical techniques for the identification of specific proteins from a complex sample mixture. This technique involves multiple processing steps and utilizes expensive reagents such as primary and secondary antibodies, and reagents for the detection of the protein bands. Another aspect of a Western blot technique is the ability to quantify the levels of specific proteins across different samples within their respective lanes. In the absence of quantification standards in the blot, this is typically accomplished by comparing the expression levels of a protein to that of a “house-keeping” protein such as Actin or Tubulin within the sample – the levels of these “house-keeping” proteins are considered to be relatively invariant between samples. However, this only provides a relative estimate of the changes of the specific protein between the samples, and not an absolute quantitative value.
“When a Western blot experiment does not produce the expected results, troubleshooting the cause of the problem can be rather challenging,” says Dr. Ali, Scientific Director and President of ADI. Absence of bands in the blot could result from one of many reasons that range from failure to include the correct antibodies or incorrect dilutions to bad reagents or degraded samples. Because of the lack of a simple and reliable method to validate every step of the process, one has to expend a significant amount of time to ascertain the cause of the problem and occasionally, discard expensive reagents.
“The TruStrip WBV product can provide all the answers and perform accurate quantification merely by including the WBV strip along with any standard Western blot,” added Dr. Ali.
The patent pending TruStrip WBV technology comprises TruStrip WBV strips and TruStrip WBV processing trays. TruStrip WBV strips are 8cm x 0.5cm in size and consist of four distinct zones containing specific reagents applied as stripes on them. The four zones include the detection reagent zone, primary antibody zone, loading/quantification zone, and secondary antibody zone. TruStrip WBV strips are processed alongside the experimental Western blot membrane and undergo the same processing steps as the experimental membrane in the same buffers. The TruStrip WBV processing trays are available as sets of detachable chambers with lids and are specifically designed to process individual, two, or three WBV strips and Western blot membrane strips, as needed. The primary benefit of the processing tray is the ability to perform incubations in minimal volume of buffers, thereby conserving usage of expensive reagents. For further details on TruStrip WBV technology and ordering details, please visit http://www.trustrips.com
About Alpha Diagnostic International, Inc.: ADI is a privately held, U.S. biotechnology company headquartered in San Antonio, Texas. ADI develops, manufactures, and supplies novel diagnostic and quality control reagents and test kits for use in basic biological and disease research in humans and animals. An important mission of ADI is to develop and supply diagnostic and validating solutions for promoting global health through improved vaccine therapeutics and mitigating the spread of human and animal diseases. For additional details, please visit: http://www.4adi.com