Yorba Linda, Ca (PRWEB) December 20, 2016
Liquid biopsies have the potential to be a less invasive method than traditional biopsies to detect advanced solid tumor mutational status. Cell free DNA (cfDNA) can be profiled from liquid biopsy samples, although the abundance is generally low, ranging from less than 1ng to 30ng cfDNA per mL blood in healthy individuals. This ctDNA is typically highly fragmented, and thus NGS-based methods to detect oncogenic driver mutations from liquid biopsies must be sensitive enough to detect low-frequency mutations from low inputs of highly fragmented material.
Anchored Multiplex PCR (AMP™) is proprietary target enrichment chemistry for NGS that is uniquely suited for highly fragmented material such as FFPE-derived nucleic acids and liquid biopsy-derived ctDNA. In this free webinar, members of the ArcherDX team will describe AMP-based ctDNA library preparation, highlighting the use and advantages of molecular barcoded adapters to unambiguously remove PCR duplicates, correct for PCR or sequencer-derived sequence errors, and flag run-to-run contamination.
Participants of the webinar can expect to gain a better understanding of both the value of mutation analysis from ctDNA in liquid biopsies and the challenges of analyzing ctDNA by next-generation sequencing. Viewers will learn how the Archer Reveal ctDNA 28 kit uses Anchored Multiplex PCR target enrichment chemistry to detect and characterize ctDNA.
LabRoots will host the webinar free for users on January 19, 2017, beginning at 8 a.m. PT, 11 a.m. ET. For more on this event, P.A.C.E and Florida continuing education credits, or to register for free, click here.
ArcherDX addresses the bottlenecks associated with using NGS in translational research by offering a robust platform for targeted sequencing applications.
By combining proprietary AMP chemistry and easy-to-use, lyophilized reagents, Archer NGS assays generate highly enriched sequencing libraries to detect gene fusions, point mutations, CNVs and RNA abundance. Complemented by the Archer suite of bioinformatics software, ArcherDX technology dramatically enhances complex mutation identification and discovery.
ArcherDX is headquartered in Boulder, Colorado.
Archer®, Reveal ctDNA™ and AMP™ are trademarks of ArcherDX, Inc.
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