Beckman Coulter Life Sciences to Showcase Analytical Methods to Measure Empty and Full AAV Particles

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LabRoots will host the webinar June 28, in which attendees will learn from a series of case studies where AUC was implemented to monitor AAV vectors analysis.

Christine LeBec, PhD Head of Analytical Development, Genethon

As part of the Beckman Coulter Life Sciences Virtual Trade Show Virtual Event, the latest in analytical methods to measure AAV particles will be discussed in a new webinar. Several examples of AAV vectors analysis will be presented, showing that AUC could be implemented as a routine test to monitor AAV product quality and manufacturing consistency.

As gene therapy approaches usually require large amounts of AAV vectors for clinical use, few manufacturing processes have been reported to provide high titer and potent quantities of products. The dose control of AAV vectors is commonly established on titration methods relying either on the quantitation of DNA or transgene expression following cell transduction.

Different indirect methods can be used to establish the ratio between full and empty AAV particles. The quantification of full capsids is performed by using qPCR based technology. The total particles can be evaluated by an ELISA assay, spectrometric analysis, ion-exchange chromatography or SDS-PAGE.

However, these methods have limitations and are not applicable to all serotypes without performing a new development. This webinar will explore those limitations, as well as methods to quantify simultaneously empty and full AAV particles as well as intermediate species containing fragmented or incomplete vector genome.

The team at Beckman Coulter Life Sciences has arranged for Dr. Christine Le Bec, head of analytical development at Genethon, to be the speaker for this webinar.

Prior to joining Genethon, Le Bec obtained her doctorate in bio-organic chemistry from the Pierre and Marie Curie University. She joined Genethon in 1997 and currently heads the analytical development department. She is responsible for developing bioassays and setting up advanced analytical tools used for characterization and in release, in-process testing of gene therapy products at early stage development.

LabRoots will host the webinar June 28, 2017, beginning at 7:00 a.m. PDT, 10 a.m. EDT. To read more about this event or to register for free, click here.

About Beckman Coulter
Beckman Coulter serves customers in two segments: Diagnostics and Life Sciences. The company develops, manufactures, and markets products that simplify, automate, and innovate complex biomedical testing. More than 275,000 Beckman Coulter systems operate in both Diagnostics and Life Sciences laboratories on seven continents. Scientists use Beckman Coulter’s Life Science research instruments to study complex biological problems, including causes of disease and potential new therapies or drugs.

About LabRoots
LabRoots is the leading scientific social networking website, which provides daily scientific trending news and science-themed apparel, as well as produces educational virtual events and webinars, on the latest discoveries and advancements in science. Contributing to the advancement of science through content sharing capabilities, LabRoots is a powerful advocate in amplifying global networks and communities. Founded in 2008, LabRoots emphasizes digital innovation in scientific collaboration and learning, and is a primary source for current scientific news, webinars, virtual conferences, and more. LabRoots has grown into the world’s largest series of virtual events within the Life Sciences and Clinical Diagnostics community.

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Karen Sorenson
LabRoots, Inc.
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