Yorba Linda, Ca (PRWEB) July 25, 2017
In this webinar, which LabRoots will host August 22, attendees will garner a better understanding of the considerations needed for designing ideal guide RNAs and DNA oligo or plasmid repair templates for HDR-mediated CRISPR-Cas9 applications.
The use of CRISPR-Cas9 to create targeted double-strand breaks in genomic DNA has greatly simplified strategies for precise gene editing using the homology-directed repair (HDR) mechanism. This approach enables knock-in of fluorescent reporters, SNP repair, exact deletions, and many other genomic alterations. However, designing target-specific guide RNAs and DNA donor templates for achieving robust cleavage and repair has many challenges. In addition, the efficiency of HDR-mediated repair is often low.
In this workshop, participants will receive a demonstration of the use of free, intuitive, online tools to design and order the necessary guide RNA and repair template reagents for HDR-mediated gene editing applications. Additionally, attendees will learn how to optimize experimental conditions to achieve robust gene editing, along with several examples of successful HDR-mediated CRISPR Cas9 experiments.
The speaker for this event will be Maren Mayer Gross, an R&D scientist at Dharmacon, a division of GE Healthcare.
Gross received her degree in biochemistry and molecular biology from the College of Wooster. She has served as an R&D scientist since joining Dharmacon in 2005. Her work has contributed to numerous research projects and new product development across RNA interference and CRISPR-Cas9 genome engineering.
LabRoots will host the event August 22, 2017, beginning at 9:00 a.m. PDT, 12:00 p.m. EDT. To read more about this event, discover the continuing education credits offered, or to register for free, click here.
GE Healthcare provides transformational medical technologies and services to meet the demand for increased access, enhanced quality, and more affordable healthcare around the world. From medical imaging, software and IT, patient monitoring, and diagnostics to drug discovery, biopharmaceutical manufacturing technologies, and performance improvement solutions, the company helps medical professionals deliver great healthcare to their patients.
LabRoots is the leading scientific social networking website, which provides daily scientific trending news and science-themed apparel, as well as produces educational virtual events and webinars, on the latest discoveries and advancements in science. Contributing to the advancement of science through content sharing capabilities, LabRoots is a powerful advocate in amplifying global networks and communities. Founded in 2008, LabRoots emphasizes digital innovation in scientific collaboration and learning, and is a primary source for current scientific news, webinars, virtual conferences, and more. LabRoots has grown into the world’s largest series of virtual events within the Life Sciences and Clinical Diagnostics community.