University of Rochester Medical Center Uses Synthego Synthetic sgRNA for CRISPR to Advance the Development and Functional Analysis of Mouse Models

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In this webinar, which LabRoots will host November 2, attendees will learn to use mouse models to study function and disease impact of coding & non-coding sequence variation.

Joe Miano, PhD Professor & Associate Director, Aab Cardiovascular Research Institute, University of Rochester

The bulk of genetic variation associated with human disease exists in the noncoding genome, much of which comprises transcribed long noncoding RNAs and non-transcribed regulatory sequences. While headway has been made in elucidating the function of some non-coding sequences, there is much to learn about how sequence variants, such as SNPs, in regulatory sequences contribute to human disease.

CRISPR/Cas-mediated genome editing offers unprecedented ease and efficiency in generating mice carrying small or large deletions as well as subtle substitutions of regulatory sequences in their native genomic milieu. Delivering synthetic single guide RNAs (sgRNA) and Cas9 protein (as RNPs) to the 1-cell mouse embryo has resulted in the rapid production of new genetic mouse models that previously would be considered risky or even challenging to engineer.

In this webinar, which is sponsored by Synthego, industry experts will explain mouse model generation using CRISPR/Cas9 and how to optimized approaches to generating, genotyping, and phenotyping ‘CRISPRized’ mice.

Data will be presented demonstrating high efficiency editing with synthetic sgRNA leading to nearly 100 percent success rate of germline transmission, driving rapid generation of mouse models. This new wave of mouse models will accelerate discovery of genomic sequence function, including the role of coding and non-coding sequence variants associated with human disease.

The speaker for this event will be Dr. Joe Miano, a professor & associate director of the Aab Cardiovascular Research Institute at the University of Rochester.

Miano received his doctorate in experimental pathology from New York Medical College and did post-doctoral training with Eric Olson at the M.D. Anderson Cancer Center in Houston. Current research in the Miano Lab focuses on deciphering how small snippets of code in our genome give cells exquisite control over the level of gene activity, much like a thermostat controlling the heat in a home.

LabRoots will host the webinar November 2, 2017, beginning 10 a.m. PDT, 1 p.m. EDT. To read more about this event, learn of the continuing education credits that will be offered, or to register for free, click here.

About Synthego
Synthego is a leading provider of genome engineering solutions. The company’s product portfolio includes software and synthetic RNA kits designed for CRISPR genome editing and research. With next-generation informatics and machine learning, Synthego’s vision is to bring precision and automation to genome engineering, enabling rapid and cost-effective research with consistent results for every scientist.

About LabRoots
LabRoots is the leading scientific social networking website, which provides daily scientific trending news, as well as produces educational virtual events and webinars, on the latest discoveries and advancements in science. Contributing to the advancement of science through content sharing capabilities, LabRoots is a powerful advocate in amplifying global networks and communities. Founded in 2008, LabRoots emphasizes digital innovation in scientific collaboration and learning, and is a primary source for current scientific news, webinars, virtual conferences, and more. LabRoots has grown into the world’s largest series of virtual events within the Life Sciences and Clinical Diagnostics community.

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Karen Sorenson
LabRoots, Inc.
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