YORBA LINDA, Calif. (PRWEB) April 30, 2018
While stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how it induces alterations in brain chemistry leading to mental illness remains poorly understood. In this webinar, the speaker will present her research findings, which focused on the novel environmentally-sensitive DNA methylation mark, 5-hydryoxymethycytosine (5hmC) which is highly enriched in the brain, sensitive to stress, and associated with active neuronal transcription.
Utilizing an optimized protocol for chromatin immunoprecipitation (ChIP) from a limited amount of brain tissue, Sisi Li generated data confirming that exposure to stress resulted in a significant decrease in the binding of a transcription factor to a candidate gene. In addition to ChIP data, Li will also present RNA-Seq and EMSA data, and describe how to prepare chromatin from low amounts of frozen brain tissue for ChIP applications.
Li will emphasize the importance of optimizing the formaldehyde fixation step of ChIP, and provide insightful tips on how to minimize tissue loss during ChIP sample preparation.
About the Presenter
Sisi Li is a neuroscience Ph.D. candidate at the University of Wisconsin, Madison. She is finishing her graduate research in the lab of Reid Alisch, PhD. Her research focuses on the role of the environmentally sensitive novel epigenetic mark, 5-hydroxymethylcytosine (5hmC) in response to stress leading to neuropsychiatric disorders.
Li joined the lab in 2013 and has three co-first author publications and seven in total over her research career. Moreover, she has given 15 oral and poster presentations within the university and at conferences across the country and is currently working on her fourth and fifth first author publications. Notably, she has collaborated with 13 different laboratories and worked closely with technical experts in three core facilities across the UW-Madison campus specializing in confocal microscopy imaging, genetic biotechnology, and flow cytometry/cell sorting.
Her final project and the last portion of her dissertation work focused on developing a deeper understanding of the molecular mechanisms linking stress, 5hmC, and transcription factor binding using chromatin immunoprecipitation (ChIP)-qPCR. Li’s ultimate goal is to transform basic science research findings into the development of personalized therapies for patients affected by stress-induced neuropsychiatric disorders.
LabRoots will host this webinar May 3, 2018, beginning at 11:00 a.m. PDT, 2:00 p.m. EDT. To learn more about this event, the speaker, or to register for free, click here.
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