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Exploring the Range of Electron Microscopes: Resolution, Size and Users, Upcoming Webinar Hosted by Xtalks


News provided by

Xtalks

Sep 11, 2024, 08:30 ET

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In this free webinar, learn about cryo-electron microscopy and the benefits of cold field emission gun (FEG) technology over traditional thermally assisted FEGs. Attendees will learn how the integration of cold FEG into 200 kV cryomicroscopes makes atomic resolution attainable on more accessible and cost-effective instruments. The featured speaker will discuss the latest advancements in cryo-EM image processing and data collection techniques. The speaker will also share how the open-source software SerialEM facilitates the automated collection of high-quality cryo-EM data.

TORONTO, Sept. 11, 2024 /PRNewswire-PRWeb/ -- The introduction of direct electron detectors significantly improved the signal-to-noise ratio of electron micrographs, resulting in a marked enhancement in the quality of cryo-electron microscope (EM) reconstructions (now referred to as the "resolution revolution").

This approach, along with advances in image processing and data collection, has pushed the resolution of single-particle cryo-EM reconstructions into the range where the electron source could become a limiting factor. This in turn has led to the reconsideration in cryo-EM of alternatives to the standard Schottky field emission gun (FEG), such as the cold FEG, owing to their superior coherence.

The familiarity that many users have with SerialEM, as well as its overall openness and ease-of-use, make obtaining high-quality data on this platform possible even for users who do not have extensive experience using a CryoARM microscope.

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Despite the known benefits of the cold FEG over thermally assisted FEG, practical limitations prevented its use in cryo-EM until JEOL's CryoARM microscope series was developed. Cold FEG was initially used on 300 kV cryomicroscopes to push the frontier of attainable resolution, ultimately resulting in cryo-EM reconstructions at "atomic resolution" where individual atoms are visible as separated spheres of density.

Following this success, attempts have been made at using the cold FEG source on 200 kV cryomicroscopes, which are generally smaller and less expensive, to see how far the tighter energy spread would allow the resolution to be pushed on this more accessible instrument.

In this webinar, the speaker will present results from a cold FEG-equipped CryoARM200 microscope that demonstrates how 200 kV data can be used to produce sub-2Å structures for a range of samples. The resolution of the reconstructions is comparable to that obtained from the more expensive state-of-the-art 300 kV microscopes.

All of the results described in the webinar are from data collected in an automated manner using the open-source software SerialEM. The familiarity that many users have with SerialEM, as well as its overall openness and ease-of-use, make obtaining high-quality data on this platform possible even for users who do not have extensive experience using a CryoARM microscope.

Register for this webinar today to gain insights into cryo-electron microscopy and how the integration of cold FEG technology in JEOL's CryoARM200 microscope is revolutionizing cryo-EM.

Join Alan Merk, Electron Microscopist, Leidos Biomedical Research, for the live webinar on Monday, September 30, 2024, at 2pm EDT (11am PDT).

For more information, or to register for this event, visit Exploring the Range of Electron Microscopes: Resolution, Size and Users.

ABOUT XTALKS

Xtalks, powered by Honeycomb Worldwide Inc., is a leading provider of educational webinars and digital content to the global life science, food, healthcare and medical device communities. Every year, thousands of industry practitioners (from pharmaceutical, biotechnology, food, healthcare and medical device companies, private & academic research institutions, healthcare centers, etc.) turn to Xtalks for access to quality content. Xtalks helps professionals stay current with industry developments, regulations and jobs. Xtalks webinars also provide perspectives on key issues from top industry thought leaders and service providers.

To learn more about Xtalks visit https://xtalks.com
For information about hosting a webinar visit https://xtalks.com/why-host-a-webinar/

Media Contact

Vera Kovacevic, Xtalks, +1 (416) 977-6555 x371, [email protected], https://xtalks.com/

SOURCE Xtalks

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