Quintara Biosciences has launched a new Nanopore-based Genotyping Validation service for the rapid confirmation of isolated single clones. By utilizing full-length amplicon sequencing (100 bp to 25 kb), the service resolves complex CRISPR edits, phases variants end-to-end, and reconstructs distinct allele sequences. Powered by a nationwide drop box network, the service delivers interactive reports and raw data files within one business day, with flexible tiers starting at $10.
BOSTON, Nov. 9, 2026 /PRNewswire-PRWeb/ -- Quintara Biosciences has launched Genotyping Validation, a full-length amplicon sequencing service for targeted genotyping of single-clone samples. Designed for PCR amplicons from 100 bp to 25 kb, the service provides full-length allele sequences to identify wild-type (WT) and edited alleles, characterize indels and other variants, and resolve complex genotypes through long-read sequencing. The service is purpose-built for allele-level confirmation of isolated single clones.
Full Length Amplicon Sequencing and Variant Phasing
By sequencing each amplicon end to end, the service captures variants across the target within individual reads. Its analysis clusters related reads to reconstruct distinct full-length allele sequences and reports estimated allele frequencies and supporting read counts. This produces a phased, allele-level representation of the edited locus.
Applications
- Wild-type, heterozygous, and homozygous clone confirmation
- CRISPR knockout, HDR/knock-in, and base-edit validation
- Detection and phasing of SNPs, indels, insertions, deletions, and multiple edits
- Reconstruction of complex alleles in cancer cell lines, polyploid or aneuploid genomes, and amplified loci
- Genotype-based sample stratification for downstream analysis
Designed Around Different Research Needs
Genotyping projects vary in allele complexity and in how researchers use the results. Quintara designed Genotyping Validation around those differences. Four service levels allow customers to match read depth and allele-reporting capacity to each sample: Basic ($10) supports up to 1,000 reads and three alleles; Standard ($30), up to 5,000 reads and six alleles; Plus ($60), up to 10,000 reads and 12 alleles; and Huge ($120), up to 20,000 reads and 24 alleles.
Results are provided in an interactive HTML report for immediate review, together with FASTA, FASTQ, allele-frequency, alignment, and per-base data files for downstream analysis. In the interactive report, researchers can compare full-length allele sequences, review estimated frequencies and supporting read counts, and inspect per-base variants and quality metrics.
Local Access and One-Day Results
Quintara's network of local drop boxes across major U.S. biotech hubs helps samples reach the lab quickly. Once samples are received, established Nanopore sequencing and bioinformatics workflows support delivery of results within one business day across all four service levels.
Nanopore Genotyping Validation service page
About Quintara Biosciences
Quintara Biosciences is a nationwide provider of genomics and molecular biology services, offering sequencing, cloning, and gene synthesis solutions for academic, biotech, and pharmaceutical clients. Quintara operates labs in Boston, San Francisco, Los Angeles, San Diego, and Houston, with an additional site in Kentucky currently under development. Quintara delivers fast, reliable, and cost-effective services designed around customer needs. www.quintarabio.com
Media Contact
Flora Zhang, Quintara Biosciences, 1 8588882878, [email protected], https://www.quintarabio.com
SOURCE Quintara Biosciences
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