Because of its fundamentally different analytical principles, LC-MS can provide qualitative and quantitative information about proteins in biological samples that is complementary to LBA results.
TORONTO (PRWEB) September 24, 2020
Over the past decade, the use of LC-MS for the quantitative determination of proteins in biological samples has increased considerably, and this technique can now be considered an established bioanalytical platform for the quantification of macromolecular drugs and biomarkers, next to the traditional ligand-binding assays (LBA’s). Because of its fundamentally different analytical principles, LC-MS can provide qualitative and quantitative information about proteins in biological samples that is complementary to LBA results.
This webinar will give an overview of recent developments in the field of protein LC-MS, as applied to support the (pre)clinical development of macromolecular pharmaceuticals. Attention will be paid to technical developments with regard to sample handling, chromatography and MS detection of both digested and intact proteins, and the relative merits of different approaches, such as immunocapture and generic extraction, or unit- and high-resolution MS, will be compared and discussed.
In addition, advances in understanding the actual meaning of the results will be highlighted. Because of the complex structure of proteins and their often neglected in vivo behavior, quite different concentration results may be obtained when different analytical techniques are applied, and a proper understanding of what a result represents is essential. The effect of protein–protein interactions, biotransformation and the occurrence of protein isoforms on the analytical result will be addressed. Practical examples from both preclinical and clinical analysis, for biopharmaceuticals and biomarkers in different biological matrices, will be used as illustrations.
For more information or to register for this event, visit Supporting the Development of Protein Drugs By LC-MS-Based Bioanalysis.
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