Cell Signaling Technology Webinar Spotlights Cross-Validation of Antibodies Using DigiWest
Yorba Linda, CA (PRWEB) September 01, 2016 -- Primary antibodies are the most crucial component of conventional western blots and DigiWest. To ensure reliable results, good antibody validation—dedicated to each application—is essential. An upcoming webinar will reveal a novel approach that integrates the principles of the western blot, namely, protein separation by SDS-PAGE, protein immobilization on a solid support, and detection by specific antibodies with a multiplexed bead array as a readout system.
Because the system can generate hundreds of bead-based western-blot equivalents from only a few micrograms of protein, users can gain information on the expression and modification of hundreds of proteins from a small sample. This new approach, which is highly reproducible, has good linearity and a large dynamic range.
Cell Signaling Technology is sponsoring a new, free educational webinar, “Cross-validation of Antibodies Using DigiWest,” which will discuss what a good antibody validation protocol means and how this varies in different applications. The scientists performed comprehensive signal transduction analysis of a lung cancer cell line that was rendered resistant to the kinase inhibitor lapatinib. In the process, hundreds of antibodies were employed, but only micrograms of protein were needed to analyze the activation state of different signaling cascades. Significant changes in the activation state of Ephrin-mediated signaling, and a central role for p53-controlled processes that form the basis of the observed resistance, could be revealed. The speakers are Anthony Couvillon, PhD, scientific marketing project manager at Cell Signaling Technology, and Markus Templin, PhD, who heads the Department of Assay Development at the NMI—Natural and Medical Sciences Institute at the University of Tuebingen, Germany.
Prior to joining Cell Signaling Technology, Dr. Couvillon was a research associate in the laboratories of Christopher L. Carpenter, MD, and Lewis Cantley, PhD, at Harvard Medical School. He received a PhD in molecular physiology and biophysics at Vanderbilt University. Dr. Couvillon later returned to Harvard and Beth-Israel Deaconess Medical Center and the Cantley lab to continue his work on Rho-family GTPases and their role in immune cell development and function, neuronal plasticity, and cardiac development. In his current role, he focuses on initiatives promoting antibody reproducibility in research.
Dr. Templin oversees a multidisciplinary environment, where scientists from applied physics, chemistry, biology, and biochemistry work in concert to develop advanced assay systems for industrial clients. Dr. Templin’s research focuses on the application of microarrays for proteomic and cancer research, and he holds several patents in this area. He studied biology at the University of Tübingen, conducted postdoctoral research at Edinburgh University, Scotland, and the Max-Planck-Institute for Developmental Biology, Germany, prior to his current position. The speakers’ complete bios are found on the LabRoots website.
The free webinar, hosted by LabRoots, will be presented on September 22, 2016, at 4 am PT/7 am ET/11 am GMT.
For full details about the event and free registration, click here.
About Cell Signaling Technology:
Cell Signaling Technology is a private, family-owned company, founded by scientists and dedicated to providing high-quality research tools to the biomedical research community. The company’s employees operate worldwide from its US headquarters in Massachusetts, and offices in the Netherlands, China, and Japan.
About LabRoots:
LabRoots is the leading scientific social networking website and producer of educational virtual events and webinars. Contributing to the advancement of science through content sharing capabilities, LabRoots is a powerful advocate in amplifying global networks and communities. Founded in 2008, LabRoots emphasizes digital innovation in scientific collaboration and learning, and is a primary source for current scientific news, webinars, virtual conferences, and more. LabRoots has grown into the world’s largest series of virtual events within the Life Sciences and Clinical Diagnostics community.
Rachel Ashton, LabRoots, Inc., http://www.LabRoots.com, +1 (530) 386-6101, [email protected]
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